Concentrations of most abundant metabolites in resting frog muscle

Range phosphocreatine ∼50 : carnosine 19.5: total free amino acids 11.7: creatine 11: total ATP 6.2 mM
Organism Frog
Reference Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6664 left column 3rd paragraphPubMed ID24901537
Primary Source [42] Godt RE, Maughan DW. On the composition of the cytosol of relaxed skeletal muscle of the frog. Am J Physiol. 1988 May254(5 Pt 1):C591-604 DOI: 10.1152/ajpcell.1988.254.5.C591PubMed ID3284380
Method Primary source abstract: "This review summarizes a variety of estimates for the concentrations of the principal cytosolic constituents in frog skeletal muscle. From these estimates (listed in the APPENDIX), [investigators] chose representative values and used electroneutrality and osmotic considerations to ensure that all major constituents have been considered. Given total cytosolic concentrations of these constituents from the literature, [they] employed a computer program to calculate the concentrations of all the major ionic species in the cytosol."
Comments P.6664 left column 3rd paragraph: "Again, these concentrations are subject to cell- and tissue-type variations. One example is frog muscle, where phosphocreatine is the most abundant metabolite (∼50 mM), followed by carnosine, total free amino acids, creatine and total ATP at respective concentrations of 19.5 mM, 11.7 mM, 11.0 mM, and 6.2 mM (primary source)."
Entered by Uri M
ID 115811