||Bacteria Escherichia coli
||Theillet FX et al., Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chem Rev. 2014 Jul 9 114(13):6661-714. doi: 10.1021/cr400695p p.6663 left column 3rd paragraphPubMed ID24901537
|| Jewett MC, Swartz JR. Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol Bioeng. 2004 Apr 5 86(1):19-26 DOI: 10.1002/bit.20026PubMed ID15007837
||P.6663 left column 3rd paragraph: "The total concentration of cytoplasmic inorganic ions in E. coli is ∼300 mM according to the CyberCell database [BNID 100124]. The concentration of K+, by far the most abundant inorganic ion, varies drastically with osmotic conditions (ref 31). 200 mM is reported to be physiologically relevant (primary source) and CyberCell notes a concentration range of 200–250 mM."