Method |
P.5 right column 2nd paragraph: "Cellular energy balance: Using constraints derived from non‐linear fittings to experimentally obtained metabolite quantification data (glucose consumption as well as acetic and lactic acid production for defining the carbon uptake and the acetic acid production, and total protein increase to restrict the growth rate) from batch culture growth (Supplementary Figure S5B–E), [investigators] applied FBA [Flux Balance Analysis] to [their] refined model to determine cellular ATP production at different growth stages (24, 36, 48, and 60 h). [They] found that during mid exponential growth (36 h after inoculation) ∼60,000 ATP molecules per cell and second are synthesized in silico (Supplementary Table S6, Supplementary Materials and methods)." |