| Reference |
Ashley E. Beck, Kristopher A. Hunt, and Ross P. Carlson, Measuring Cellular Biomass Composition for Computational Biology Applications, Processes 2018, 6(5), 38, doi:10.3390/pr6050038 p.18 table 6 |
| Method |
P.18 top paragraph: "The following steps convert experimentally measured mass fractions of macromolecules to molar coefficients for use in the biomass reaction: (1) Record mass fractions as g macromolecule per g cell dry weight (see Table 4). (2) Tabulate the molar mass of each macromolecule representation. Multiply the macromolecular formula by the atomic mass of the respective elements, and sum over all elements to obtain g/mol macromolecule. (3) Divide the mass fraction of the macromolecule by its molar mass to obtain mol macromolecule/g cell dry weight. The basis for cell dry weight normalization can be selected as desired 1, 10, or 100 kg cell dry weight typically results in reasonably scaled coefficients for elementary flux mode and flux balance analyses. One kilogram cell dry weight often provides a convenient basis, as when inputs are scaled to a mM basis in FBA [Flux Balance Analysis], the resulting output biomass scales to grams. (4) Incorporate the molar coefficients into the biomass reaction. The stoichiometries can be multiplied by the macromolecular formulas and summed over all the macromolecules to obtain an overall formula for biomass, which allows model output to be analyzed in terms of carbon moles of biomass (Table 6)." |