| Method |
P.567 caption to table 1: "After treatment with 100 nM T3 [triiodothyronine] for 24 h, cells were incubated with
1 μg/mL actinomycin D and 100 nM T3 for 1, 2, 4, or 6 h followed
by extraction of total RNA and reverse transcription. Quantitative
PCR was performed to determine mRNA expression levels. The data
relative to the values obtained from cells treated for various times
were plotted. The mRNA half-life was calculated from the formula
obtained by linear regression using the least-squares method. The
experiments were repeated 4 times to calculate the means ± SD." |