| Method |
P.202 right column 2nd paragraph: "In conclusion, [investigators] provide a comprehensive view of protein abundance in yeast by normalizing and combining 21 abundance datasets, collected by MS [Mass Spectrometry], GFP fluorescence flow cytometry, GFP fluorescence microscopy, and western blotting. Since cellular autofluorescence interferes with detection of GFP fluorescence, [they] find that the lower limit for reliable detection of GFP proteins corresponds to ∼1,400 molecules per cell. Above this threshold [they] found less variation among GFP-based studies than among MS studies, suggesting that although MS analyses provide the greatest sensitivity and dynamic range for protein measurements, the GFP-based measurements have greater precision." |