Number of ribosomes
Value | 4500 ribosomes/cell Range: ±500 ribosomes/cell |
---|---|
Organism | Bacteria Leptospira interrogans |
Reference | Beck M, Malmström JA, Lange V, Schmidt A, Deutsch EW, Aebersold R. Visual proteomics of the human pathogen Leptospira interrogans. Nat Methods. 2009 Nov6(11):817-23. DOI: 10.1038/nmeth.1390 p.819 figure 2b, left column top paragraph, p.820 table 1 and supplementary material p.12 supplementary table 1PubMed ID19838170 |
Method | P.818 right column bottom paragraph: "[Investigators] therefore measured the absolute average cellular concentration of the targeted protein complexes under all the investigated conditions by selected reaction monitoring (SRM)–mass spectrometry [ref 13] to generate test datasets containing the target protein complexes at their actual cytoplasmic concentration and to validate the signals derived from real data with an independent method (Supplementary Table 2). The number of copies per cell of the targeted complexes ranged from 4,500 copies per cell for the ribosome in nonstimulated cells to 15 copies per cell HslU-HslV complex in antibiotic-treated cells (Table 1)." |
Entered by | Uri M |
ID | 114930 |