Typical stromal HCO3– concentration apparently required for CCM (CO2 concentrating mechanism) function

Range 2 – 3 mM
Organism Green algae Chlamydomonas reinhardtii
Reference Wang Y, Stessman DJ, Spalding MH. The CO2 concentrating mechanism and photosynthetic carbon assimilation in limiting CO2 : how Chlamydomonas works against the gradient. Plant J. 2015 May82(3):429-48. doi: 10.1111/tpj.12829 p.444 left column bottom paragraphPubMed ID25765072
Primary Source Badger MR, Kaplan A, Berry JA. Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM. Plant Physiol. 1980 Sep66(3):407-13 AND Duanmu D, Miller AR, Horken KM, Weeks DP, Spalding MH. Knockdown of limiting-CO2-induced gene HLA3 decreases HCO3- transport and photosynthetic Ci affinity in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2009 Apr 7 106(14):5990-5. doi: 10.1073/pnas.0812885106 AND Moroney JV, Tolbert NE. Inorganic Carbon Uptake by Chlamydomonas reinhardtii. Plant Physiol. 1985 Feb77(2):253-8 AND Spalding MH, Spreitzer RJ, Ogren WL. Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth. Plant Physiol. 1983 Oct73(2):268-72 AND Spalding MH, Spreitzer RJ, Ogren WL. Reduced Inorganic Carbon Transport in a CO(2)-Requiring Mutant of Chlamydomonas reinhardii. Plant Physiol. 1983 Oct73(2):273-6PubMed ID16661446, 19321421, 16664038, 16663206, 16663207
Comments P.444 left column bottom paragraph: "The eukaryotic algal CCM may have unique advantages for transferring a CCM to higher plants, because eukaryotic algae and vascular plants share close evolutionary relationships and similar photosynthetic and many other molecular characteristics. The typical 2–3 mM stromal HCO3– concentration apparently required for microalgal CCM function (primary sources) may also be much easier to achieve than the 20–40 mM internal HCO3– concentration characteristic of the typical cyanobacterial system (BNID 114620)."
Entered by Uri M
ID 114619