| Comments |
Abstract: "The rate of dissociation of the complex is very slow and follows first-order kinetics with a half-life of 19 minutes in [investigators'] standard buffer (0.05 M ionic strength)." P.404 4th paragraph: "In Figure 2, [investigators] have concentrated on the initial linear portion of the curve, and it can be seen that first-order kinetics are followed for at least 30 minutes, with the halflife being close to 19 minutes...In [their] standard BB buffer at 24°C the half-life is 19±4 minutes, where the range is the standard deviation for 18 experiments." P.402 4th paragraph: "BB buffer contains: 0.01 M-magnesium acetate, 0.01 M-KCl, 10^-4 M-EDTA, 10^-4M-dithiothreitol, 5% dimethyl sulfoxide, 0.01 M-Tris-HCl, pH 7.4 at 24˚C, and 50 µg bovine serum albumin/ml." |