| Value |
45
base pairs
Range: ±10 base pairs
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Hammar P et al. The lac Repressor Displays Facilitated Diffusion in Living Cells. Science. 2012 June 22 336(6088):1595-1598. doi: 10.1126/science.1221648 abstract, p.1598 right column bottom paragraph and middle column bottom paragraphPubMed ID22723426
|
| Method |
P.1595 right column 2nd paragraph: "Using a yellow fluorescent protein–labeled LacI in Escherichia coli cells, [investigators] developed an assay for measuring the search time based on the distinction between localized and diffuse fluorescence signals (ref 14)." |
| Comments |
Abstract: "Here [investigators] show that the lac repressor slides 45±10 base pairs on chromosomal DNA and that sliding can be obstructed by other DNA-bound proteins near the operator." P.1598 right column bottom paragraph and middle column bottom paragraph: link |
| Entered by |
BC2333 |
| ID |
114239 |