| Range |
33±4 %
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Makarova M et al., Temporal Regulation of Lipin Activity Diverged to Account for Differences in Mitotic Programs. Curr Biol. 2016 Jan 25 26(2):237-43. doi: 10.1016/j.cub.2015.11.061 p.239 left column 2nd paragraphPubMed ID26774782
|
| Method |
Abstract: "Eukaryotes remodel the nucleus during mitosis using a variety of mechanisms that differ in the timing and the extent of nuclear envelope (NE) breakdown. Here, [investigators] probe the principles enabling this functional diversity by exploiting the natural divergence in NE management strategies between the related fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus [refs 1-3]. [Investigators] show that inactivation of Ned1, the phosphatidic acid phosphatase of the lipin family, by CDK [Cyclin-dependent kinases] phosphorylation is both necessary and sufficient to promote NE expansion required for "closed" mitosis in S. pombe." |
| Comments |
P.237 left column 2nd paragraph: "The surface area of a mother nucleus undergoing closed mitosis must increase to allow intranuclear mitotic spindle elongation and formation of the daughter nuclei. The model yeasts S. pombe and Saccharomyces cerevisiae solve this problem through nuclear envelope (NE) expansion at mitotic entry [refs 1, 4–9]. In contrast, S. japonicus, an S. pombe relative, does not expand its NE and instead relies on NE breakdown during anaphase to allow chromosome segregation [refs 1, 3]." P.239 left column 2nd paragraph: "Indeed, unlike in the wild-type cells where the NE surface area grew by 33% ± 4% during mitosis (n = 20 cells, see also [ref 1]), the Ned[1S627E/S629E] mutant cells showed only a modest 12% ± 5% increase (n = 20, Figures 3B and 3C)." |
| Entered by |
Uri M |
| ID |
114004 |