| Value |
50
%
|
| Organism |
Human Homo sapiens |
| Reference |
Yewdell JW, Reits E, Neefjes J. Making sense of mass destruction: quantitating MHC class I antigen presentation. Nat Rev Immunol. 2003 Dec3(12):952-61. DOI: 10.1038/nri1250 p.958 left column bottom paragraphPubMed ID14647477
|
| Primary Source |
[62] Siffroi-Fernandez, S., Giraud, A., Lanet, J. & Franc, J. L. Association of the thyrotropin receptor with calnexin, calreticulin and BiP. Efects on the maturation of the receptor. Eur. J. Biochem. 269, 4930–4937 (2002).PubMed ID12383251
|
| Method |
Primary source abstract: "In this study, [investigators] investigated whether calnexin (CNX), calreticulin (CRT) and BiP, three of the main molecular chaperones present in the endoplasmic reticulum, interact with the TSHR [thyrotropin receptor] and what effects these interactions might have on the folding of the receptor. In the first set of experiments, [they] observed that in a K562 cell line expressing TSHR, about 50% of the receptor synthesized was degraded by the proteasome after ubiquitination." Primary source studied K562 cells - human immortalised myelogenous leukemia cells |
| Comments |
P.958 left column 3rd paragraph: "Fifty percent of thyrotropin receptor expressed from a transfected gene is destroyed rapidly by proteasomes [primary source], which also destroy 60% of transgene-expressed epitope-tagged delta-opioid receptor [BNID 113779]." |
| Entered by |
Uri M |
| ID |
113778 |