Characteristics of the genome

Range Table - link
Organism Tree Norway Spruce Picea abies
Reference Nystedt B et al., The Norway spruce genome sequence and conifer genome evolution. Nature. 2013 May 30 497(7451):579-84. doi: 10.1038/nature12211 p.580 table 1PubMed ID23698360
Method P.579 right column bottom paragraph: "De novo sequencing and assembly of large, repeat-containing, heterozygous genomes remains challenging. To assemble the P. abies genome, [investigators] developed a hierarchical strategy combining fosmid pools (ref 11) with both haploid and diploid whole genome shotgun (WGS) data, and RNA sequencing (RNA-Seq) data (refs 12–14) (Supplementary Information 1.2–1.3).The resulting assembly (P.abies 1.0) included 4.3 Gb in >10-kilobase (kb) scaffolds (Table 1), and [they] estimated that approximately 63% of protein-coding genes (ref 15) were fully covered (>90% of their length), and 96% partially covered (>30% of their length) within single scaffolds (Supplementary Information 1.4)."
Comments P.580 right column bottom paragraph: "[Investigators] constructed a manually curated library of 1,773 repetitive sequences, approximately half of which could be assigned to known transposable element repeat families (Supplementary Information 3.1–3.3). Long terminal repeat-retrotransposons (LTR-RTs) comprised the most abundant fraction of transposable elements, with the Ty3/Gypsy superfamily being more abundant than the Ty1/Copia superfamily (Fig. 2a and Table 1)."
Entered by Uri M
ID 113649