| Value |
3
%
|
| Organism |
Human Homo sapiens |
| Reference |
Aravinthan A. Cellular senescence: a hitchhiker's guide. Hum Cell. 2015 Apr28(2):51-64. doi: 10.1007/s13577-015-0110-x p.53 left column 2nd paragraphPubMed ID25690721
|
| Primary Source |
[40] Chen Q, et al. Oxidative DNA damage and senescence of human diploid fibroblast cells. Proc Natl Acad Sci USA. 1995 92(10):4337–41.PubMed ID7753808
|
| Method |
Primary source abstract: "To address the cause of growth cessation in senescent IMR-90 human fibroblast cells, [investigators] determined the level of oxidative DNA damage by using 8-oxoguanine excised from DNA and 8-oxo-2'-deoxyguanosine in DNA as markers." |
| Comments |
P.53 left column 2nd paragraph: "Oxidative stress, through accumulation of intracellular ROS [reactive oxygen species], causes oxidative damage to DNA and proteins [refs 37-39] and plays an important role in the induction of senescence. Induction of senescence in human fibroblasts was delayed significantly when they were cultured in more physiological tissue oxygen concentration (3 %) compared to conventional tissue culture oxygen concentrations (20 %) [primary source]. Treatment with sub-lethal concentrations of hydrogen peroxide has been shown to induce senescence in a variety of human cells [refs 37, 41- 47] making this an attractive in vitro model for the study of cellular senescence." |
| Entered by |
Uri M |
| ID |
113607 |