Maximum cleavage rate of hammerhead ribozyme (HHR)

Range ~1,000 min^-1
Organism Trematode Schistosoma mansoni
Reference de la Peña M, García-Robles I, Cervera A. The Hammerhead Ribozyme: A Long History for a Short RNA. Molecules. 2017 Jan 4 22(1). pii: E78. doi: 10.3390/molecules22010078 p.5 top paragraphPubMed ID28054987
Primary Source [10] Emilsson GM, Nakamura S, Roth A, Breaker RR. Ribozyme speed limits. RNA. 2003 Aug9(8):907-18.PubMed ID12869701
Comments P.5 top paragraph: "Moreover, changes in the loop sequences induced a large reduction in the cleavage rate (<0.01/min) [ref 44], suggesting that steric clashes prevented the necessary and specific interactions for proper folding of the ribozyme. Similar results were obtained for other HHRs, including the type I HHR encoded in the satellite DNA Smα of the Schistosoma mansoni trematode [ref 46], which reached a maximum cleavage rate close to 1000/min [primary source]. Moreover, detailed kinetic analysis of the full S. mansoni HHR also revealed a 2000-fold increase in the rate of ligation compared to minimal hammerheads without tertiary interactions [ref 47]."
Entered by Uri M
ID 113525