Copy number of 2μ plasmid

Range 40 - 80 copies per haploid genome
Organism Budding yeast Saccharomyces cerevisiae
Reference Gnügge R, Rudolf F. Saccharomyces cerevisiae Shuttle vectors. Yeast. 2017 May34(5):205-221. doi: 10.1002/yea.3228 p.208 right column 2nd paragraph & p.210 right column 3rd paragraphPubMed ID28072905
Primary Source Clark-Walker GD, Miklos GL. 1974. Localization and quantification of circular DNA in yeast. Eur J Biochem 41: 359–365. AND Futcher AB, Cox BS. 1984. Copy number and the stability of 2-micron circle-based artificial plasmids of Saccharomyces cerevisiae. J Bacteriol 157: 283–290 AND Gerbaud C, Guerineau M. 1980. 2 micron plasmid copy number in different yeast strains and repartition of endogenous and 2 micron chimeric plasmids in transformed strains. Curr Genet 1: 219–228 DOI: 10.1007/BF00390947PubMed ID4593580, 6361000, 24189662
Comments P.208 right column 2nd paragraph: "Yeast episomal plasmids (YEps) are based on sequences from a natural yeast plasmid. This plasmid is present in most wild-type and laboratory S. cerevisiae strains and has a length of 6318 bp (Hartley and Donelson, 1980). Alluding to its contour size, it was termed 2μm or 2μ plasmid (Stevens and Moustacchi, 1971, Guerineau et al., 1971). The plasmid is cryptic, as it is not associated with any apparent phenotype and confers no selective advantage to its host cell, nevertheless, its loss is very rare (Futcher and Cox, 1983). The 2μ plasmid persists in yeast cells with 40–80 copies per haploid genome (primary sources). The plasmid copies are not homogeneously distributed in the nucleus, but are found in a few clusters (Scott-Drew and Murray, 1998, Velmurugan et al., 2000)." P.210 right column 3rd paragraph: "YEps containing the complete 2μ sequence achieve 40–80 copies per cell, which is comparable to the endogenous 2μ plasmid copy number (primary source Futcher and Cox, 1984)."
Entered by Uri M
ID 113493