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P.16 left column bottom paragraph: "Thus the highly-purified mitochondria were subjected to different separation techniques (1D, 2D PAGE [polyacrylamide gel electrophoresis] and multidimensional liquid chromatography) and proteolytic digestion with different enzymes prior to LC-MS [Liquid chromatography–mass spectrometry]. In 2006, this first comprehensive yeast mitochondrial proteome was further expanded through the use of more advanced LC [liquid chromatography] and LC-MS methods by Reinders et al. (2006), leading to the identification of 102 additional proteins and estimating that the total of 851 identified proteins would correspond to 84% of the mitochondrial proteome in the MITOP [mitochondria-related
proteins] yeast database (Scharfe et al., 1999), which would then amount to 1000 proteins. As of May 2016, a total of 1187 genes in the Saccharomyces Genome Database (SGD) (Cherry et al., 2012) were assigned to mitochondria (see Fig. 1)." P.16 caption to figure 1: "The 1187 proteins that were assigned to mitochondria in SGD as of May 2016 were classified, based on Gene Ontology Terms. As in the first mitochondrial proteome from Sickmann et al. (primary source) with 749 proteins, the share of proteins that are related to maintenance and expression of the mitochondrial genome is surprisingly high (22% compared to 25%), whereas the share of proteins with still (rather) unknown function decreased from 25% to 10%." |