| Comments |
P.276 right column bottom paragraph: "The E. coli data described above are summarized in Table 1. In the few confirmed examples, misreading errors seem to occur most frequently at the first or third position of the codon and can involve both pyrimidines and purines. Second-
position errors may also occur. Some errors clearly occur at or above a frequency of 10^-3 in normally growing cells. It is possible that some of these high levels, such as the misreading of CGU, are context dependent, but this seems not to be the case with AAU. Many errors occur at a level closer to 10^-4, and misreading at many codons has so far been undetectable. It seems most likely that the average
misreading error frequency may be well below 10^-3 per codon. Nonetheless, some misreading occurs at frequencies well above this, and some particular tRNAs may be error prone, perhaps those for cysteine, lysine, and glutamine." |