| Comments |
P.3503 right column bottom paragraph: "The Muscle Proteome Is Quantitatively Dominated by Large, Abundant Proteins: As a practical example of the usefulness of “easy” absolute protein quantification, [investigators] determined cell sizes and cellular copy numbers of proteins in a panel of other mouse organs (Fig. 6A). Ovaries consist predominantly of small follicular cells and showed the least protein per cell (42 pg). Leg muscle cells, in contrast, had around 675 pg of protein per nucleus. Considering that muscle fibers are syncytial, multi-nucleated cells, the histone proteomic ruler delivered protein amounts per nucleus and not per cell in this particular case. Despite the huge differences in cellular protein amounts, [they] observed much less variation in the dependence of the abundance of a protein and its molecular mass, irrespective of the tissue of origin." |