| Value |
2.3
kb/min
Range: previous estimates 1.6-3kb/min kb/min
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Gispan A, Carmi M, Barkai N. Model-based analysis of DNA replication profiles: Predicting replication fork velocity and initiation rate by profiling free-cycling cells. Genome Res. 2016 Dec 27. pii: gr.205849.116. doi: 10.1101/gr.205849.116 p.5 top paragraph link PubMed ID28028072
|
| Primary Source |
Sekedat MD, Fenyo D, Rogers RS, Tackett AJ, Aitchison JD, Chait BT. 2010. GINS motion reveals replication fork progression is remarkably uniform throughout the yeast genome. Mol Syst Biol 6: 353. link DOI: 10.1038/msb.2010.8 AND Yang SC, Rhind N, Bechhoefer J. 2010. Modeling genome-wide replication kinetics reveals a mechanism for regulation of replication timing. Mol Syst Biol 6: 404. link DOI: 10.1038/msb.2010.61 AND Hyrien O, Goldar A. 2010. Mathematical modelling of eukaryotic DNA replication. Chromosom Res 18: 147–161. link doi: 10.1007/s10577-009-9092-4 AND Yabuki N, Terashima H, Kitada K. 2002. Mapping of early firing origins on a replication profile of budding yeast. Genes Cells 7: 781–789. link AND Raghuraman MK et al., 2001. Replication dynamics of the yeast genome. Science (80- ) 294: 115–121. link DOI: 10.1126/science.294.5540.115 PubMed ID20212525, 20739926, 20205354, 12167157, 11588253
|
| Method |
P.5 top paragraph: "Validating predicted changes in fork velocity and initiation capacity using time-resolved profiling: [Investigators] next wished to verify predicted changes in fork velocity or initiation capacity. This required an independent means for measuring these parameters. To this end, [they] profiled cells that progress synchronously through S phase. Cells were arrested at the end of G1 using α-factor, and were followed for sixty minutes upon release, with samples taken every three minutes for DNA sequencing. Synchronized progression was verified by DNA staining (Fig. 4A). Plotting DNA content as a function of chromosomal coordinates at different times showed the expected v-shape increase in DNA content around replication origins, capturing the symmetric progression of the replication fork (Fig. 4B)." |
| Comments |
P.5 top paragraph: "Based on this pattern around well-characterized origins [see Measurement Method], [investigators] estimated wild-type fork velocity to be 2.3 kb/min, consistent with previous estimates (1.6-3 kb/min)(primary sources)." |
| Entered by |
Uri M |
| ID |
113067 |