Synthesis rate of RBCL [Rubisco plastid-encoded large subunit] and RBCS [Rubisco nucleus-encoded small subunit] in the light period and the night

Range Table - link
Organism Thale cress Arabidopsis thaliana
Reference Ishihara H, Obata T, Sulpice R, Fernie AR, Stitt M. Quantifying protein synthesis and degradation in Arabidopsis by dynamic 13CO2 labeling and analysis of enrichment in individual amino acids in their free pools and in protein. Plant Physiol. 2015 May168(1):74-93. doi: 10.1104/pp.15.00209. p.83 table IIIPubMed ID25810096
Method See note above table
Comments P.82 left column bottom paragraph: "Synthesis rates were lower at night but similar for RBCL and RBCS (0.37 and 0.36% h^-1, respectively Table III)." P.88 left column bottom paragraph: "The pgm [starchless phosphoglucomutase] mutant has an approximately 40% smaller daily net increment in protein than wild-type Col-0 (11.9%–13.7% d^−1 in pgm compared with 20.5%–22.1% d^−1 in Col-0). This closely matches the observed decrease in growth (38%) estimated either from size measurements or from Glc incorporation into the cell wall. This decrease in net protein synthesis is the result of a 29% reduction in the amount of protein synthesized per 24-h cycle, due to the lower rate of protein synthesis in the night, and a 26% to 35% increase in the rate of protein degradation (Table III)." Col-0=Columbia-0 plant
Entered by Uri M
ID 112884