Estimates of actin filament concentrations in lamellipodia

Range 700-1,600 μM
Organism Mammals
Reference Koestler, S.A., K. Rottner, F. Lai, J. Block, M. Vinzenz, and J.V. Small. 2009. F- and G-actin concentrations in lamellipodia of moving cells. PLoS ONE. 4:e4810. doi:10.1371/journal.pone.0004810 p.1 right column top paragraphPubMed ID19277198
Primary Source [18] Hoglund AS, Karlsson R, Arro E, Fredriksson BA, Lindberg U (1980) Visualization of the peripheral weave of microfilaments in glia cells. J Muscle Res Cell Motil 1: 127–146. [19] Abraham VC, Krishnamurthi V, Taylor DL, Lanni F (1999) The actin-based nanomachine at the leading edge of migrating cells. Biophys J 77: 1721–1732. DOI:10.1016/S0006-3495(99)77018-9PubMed ID6894451, 10465781
Method See comments section
Comments P.1 right column top paragraph: "Estimates of actin filament concentrations in lamellipodia range from 700 µM, based on filament counts from electron microscopy [primary source 18] to 1600 µM, derived from the comparison of the phalloidin label intensities of single filaments and lamellipodia of fixed cells [primary source 19]. The latter authors concluded that the G-actin concentration at the lamellipodium tip was in the range of 8 µM, based on in vitro rate constants for polymerization [primary source 19]." Primary source [18] studied human glia cells. Primary source [19] studied mice 3T3 fibroblasts.
Entered by Uri M
ID 112789