| Range |
red cell average hemoglobin concentration 14.8g/dL: average hematocrit 42ml/dL
|
| Organism |
Human Homo sapiens |
| Reference |
Silverman L, Glick D. Measurement of protein concentration by quantitative electron microscopy. J Cell Biol. 1969 Mar40(3):773-8. p.776 left column top paragraphPubMed ID5765765
|
| Method |
Abstract: "The method of quantitative electron microscopy was applied to the measurement of protein concentration in thin sections. The human erythrocyte was selected as a model because of its apparently uniform protein concentration. Phosphotungstic acid (PTA) in aqueous solution was used as a reversible stain for protein, and PTA-stained Dowex resin spheres were embedded along with the red cells as standards for measurement of section thickness. The mass of stain removed from a given area of sectioned red cell by buffer (pH 7.4) was measured by quantitative electron microscopy. From the stoichiometry of the reaction between PTA and red cell protein established in this study, the amount of protein present in the measured area was calculated." |
| Comments |
P.776 left column top paragraph: "Red Cell Hemoglobin Concentration. The average hemoglobin concentration was 14.8 g per 100 ml of blood and the average hematocrit value was 42 ml per 100 ml. From these data the concentration was calculated to be 35.2 g per 100 ml of packed red cells." |
| Entered by |
Uri M |
| ID |
112778 |