| Range |
2×10^6 - 4×10^6 M^-1×sec^-1
|
| Organism |
Mouse Mus musculus |
| Reference |
Northrup SH, Erickson HP. Kinetics of protein-protein association explained by Brownian dynamics computer simulation. Proc Natl Acad Sci U S A. 1992 Apr 15 89(8):3338-42. p.3338 left column bottom paragraphPubMed ID1565624
|
| Primary Source |
[5] Ward, E. S., Gussow, D., Griffiths, A. D., Jones, P. T. & Winter, G. (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature (London) 341, 544-546.PubMed ID2677748
|
| Method |
Primary source [5] abstract: "Using the polymerase chain reaction, diverse libraries of VH [heavy variable] genes were cloned from the spleen genomic DNA of mice immunized with either lysozyme or keyhole-limpet haemocyanin. From these libraries, VH domains were expressed and secreted from Escherichia coli." |
| Comments |
p.3338 left column bottom paragraph: "The binding of Fab [fragment antigen-binding] fragments and recombinant domains of antibody D1.3 to its antigen occurs with k2=2-4×10^6M^-1×s^-1 (primary source)." |
| Entered by |
Uri M |
| ID |
112539 |