| Range |
~10 µm/sec
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Upadhyaya A, Sheetz MP. Tension in tubulovesicular networks of Golgi and endoplasmic reticulum membranes. Biophys J. 2004 May86(5):2923-8. p.2927 right column bottom paragraphPubMed ID15111408
|
| Primary Source |
Sciaky N et al., Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J Cell Biol. 1997 Dec 1 139(5):1137-55.PubMed ID9382862
|
| Method |
Primary source abstract: "Here, [investigators] use green fluorescent protein-Golgi protein chimeras to study Golgi morphology in vivo." |
| Comments |
P.2927 right column bottom paragraph: "Is the observed tension difference [BNID 112519] sufficient to cause membrane flow from Golgi to ER as observed after addition of BFA [drug Brefeldin A] (Lippincott-Schwartz et al., 1989, 1990)? From the measured redistribution of a fluorescent Golgi protein into the ER, Sciaky et al. (primary source) have concluded that movement of membrane protein between Golgi and ER is due to convective flow rather than diffusive movement, with velocities on the order of 10 μm/s." Primary source investigated HeLa and CHO cells |
| Entered by |
Uri M |
| ID |
112520 |