Effect of sugars on lipid lateral mobility in GUVs (giant unilamellar vesicles)

Range Table - link 10^-8 cm^2/s
Organism Unspecified
Reference Doeven MK et al., Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles. Biophys J. 2005 Feb88(2):1134-42. p.1140 table 2PubMed ID15574707
Method P.1139 right column top paragraph:"To investigate the effect of high concentrations of sucrose on the lateral mobility of lipids, [investigators] prepared GUVs in the absence and presence of high concentrations of sugar. Although the efficiency of GUV formation was lowered when sugar was present in the rehydration liquid, GUVs could still be formed with up to 1.5 M sucrose (or glucose) in the rehydration medium (Fig. 6, A and B). Representative autocorrelation curves for the fluorescent lipid probe DiO in GUVs prepared in water or 1.5 M sugar are shown in Fig. 6 C."
Comments P.1139 right column top paragraph:"It can be seen that at a high sucrose concentration, the lateral diffusion of DiO was slowed by a factor of ∼3. This suggests that the sugar is binding to the lipids, and cluster formation may be taking place. When glucose was used instead of sucrose, the effect on the lateral mobility of DiO was much smaller, but still a reduction of ∼2-fold was observed. In Table 2, a summary of the measured diffusion coefficients is given. Control experiments with another fluorescent lipid analog (NBD C6-HPC Molecular Probes) gave similar results (not shown), indicating that the results were not probe-specific." See note beneath table. FCS=fluorescence correlation spectroscopy
Entered by Uri M
ID 112265