Fraction of CCPs [Clathrin-Coated Pits] that have no dynamin recruitment in RPE [retinal pigment epithelium] cells

Range ~50 %
Organism Human Homo sapiens
Reference Aguet F, Antonescu CN, Mettlen M, Schmid SL, Danuser G. Advances in analysis of low signal-to-noise images link dynamin and AP2 to the functions of an endocytic checkpoint. Dev Cell. 2013 Aug 12 26(3):279-91. doi: 10.1016/j.devcel.2013.06.019. p.286 left column bottom paragraphPubMed ID23891661
Method P.290 left column 2nd paragraph:"The procedures used for culture of human RPE cells, siRNA transfection, CHC [clathrin heavy chain] immunoprecipitation, and immunoblotting are described in the Supplemental Experimental Procedures available online." 3rd paragraph:"TIRF [Total internal reflection fluorescence] microscopy"
Comments P.286 left column bottom paragraph:"Unlike the report by Doyon et al. (2011), [investigators] also identified a large fraction of dynamin-deficient CCPs (∼50%). [They] compared the lifetime distributions of CCPs with or without dynamin and found significant differences both in their median lifetime (∼65 s versus ∼30 s) and shape (Figure 5E for the full decomposition of lifetimes, see Figure S4B). Thus, in the absence of dynamin, the disappearance of CCPs from the plasma membrane is dramatically accelerated. More importantly, the lifetime distribution for dynamin-deficient CCPs displayed a quasi-exponential shape characteristic of a process where the disassembly of structures is triggered by a single random event that occurs at a specific rate (Figure S3A), indicative of abortive events."
Entered by Uri M
ID 112148