| Range |
~40 sec
|
| Organism |
Monkey |
| Reference |
Mettlen M, Danuser G. Imaging and modeling the dynamics of clathrin-mediated endocytosis. Cold Spring Harb Perspect Biol. 2014 Aug 28 6(12):a017038. doi: 10.1101/cshperspect.a017038. p.9 right column bottom paragraphPubMed ID25167858
|
| Primary Source |
Ehrlich M. et al., 2004. Endocytosis by random initiation and stabilization of clathrin-coated pits. Cell 118: 591–605. & Loerke D. et al., 2009. Cargo and dynamin regulate clathrin-coated pit maturation. PLoS Biol 7: e57 doi: 10.1371/journal.pbio.1000057.PubMed ID15339664, 19296720
|
| Method |
1st primary source abstract:"[Investigators] have detected the formation of coats by monitoring incorporation of fluorescently tagged clathrin or its adaptor AP-2 [they] have also followed clathrin-mediated uptake of transferrin and of single LDL [Low-density lipoprotein] or reovirus particles." 2nd primary source abstract:"Total internal reflection fluorescence microscopy (TIR-FM)" |
| Comments |
P.9 right column bottom paragraph:"Two of the first imaging studies aiming at a representative sampling of CCP lifetimes revealed broad distributions with a large fraction of the population living <20 sec but also a significant fraction living for >90 sec (Fig. 4A) (primary sources). Using the same mammalian BSC-1 cell model, both studies also agreed on the mean lifetime of ∼40 sec and in the observation of a characteristic mode of preferred lifetime between 20 and 30 sec. This suggested that CME [Clathrin-mediated endocytosis] is a regulated process with a major decision in the first 30 sec as to whether CCPs terminate early or continue to live for up to 2–3 min. Importantly, this lifetime distribution stands in stark contrast to a distribution with an exponential decay (Fig. 1A), which would either represent an erroneous measurement, or suggest that CCP internalization after assembly is a random process following first-order kinetics." |
| Entered by |
Uri M |
| ID |
112147 |