Number of CCSs [clathrin-coated structures] in RPE [retinal pigment epithelium] cells

Range ~9,000±3,000 CCSs/cell
Organism Human Homo sapiens
Reference Aguet F, Antonescu CN, Mettlen M, Schmid SL, Danuser G. Advances in analysis of low signal-to-noise images link dynamin and AP2 to the functions of an endocytic checkpoint. Dev Cell. 2013 Aug 12 26(3):279-91. doi: 10.1016/j.devcel.2013.06.019. p.282 caption to figure 2APubMed ID23891661
Method P.290 left column 2nd paragraph:"The procedures used for culture of human RPE cells, siRNA transfection, CHC [clathrin heavy chain] immunoprecipitation, and immunoblotting are described in the Supplemental Experimental Procedures available online." 3rd paragraph:"TIRF [Total internal reflection fluorescence] microscopy"
Comments P.281 left column 2nd paragraph:"Equipped with this detection algorithm, [investigators] first determined the lifetime distributions of all detected clathrin structures (CSs) in EGFP-CLCa O/X cells (Figure 2A)." P.282 caption to figure 2A:"Lifetime distribution of detected CCSs from ten cells overexpressing EGFP-CLCa (colored traces, individual cells black trace, population average)~9,000 ± 3,000 CCSs per cell." CLC=clathrin light chains. EGFP-CLCa O/X cells=EGFP-CLCa fusions
Entered by Uri M
ID 112144