| Comments |
P.539 right column:"To make sure that the observed changes in lysosomal displacement were not corrupted by changes in cell shape (e.g., cell swelling, cell shrinking, or changes in cell polarity or spreading), cell size was characterized at different pH values by determining the maximum lysosome-to-nucleus distance. This maximum distance, obtained from lysosomes localized at the plasma membrane, was used as an index for alterations in cell size at different pH values. The results of these measurements are summarized in Table 1. No significant differences of these values were detected in the HMECs or any of the breast cancer cells under different pH conditions, demonstrating that there were no changes in cell shape with extracellular acidosis." Normal human
mammary epithelial cells (HMECs) and human breast cancer cells. Keywords:size,organelle,hydrolysis,degradation,endocytic pathway |