| Range |
~50-75% of the cytoplasmic volume: ~2-3% of the space in which it is present
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Wingreen NS, Huang KC. Physics of Intracellular Organization in Bacteria. Annu Rev Microbiol. 2015 Oct 15 69: 361-79. doi: 10.1146/annurev-micro-091014-104313. p.364 bottom paragraphPubMed ID26488278
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| Primary Source |
[6] Bakshi S, Siryaporn A, Goulian M, Weisshaar JC. 2012. Superresolution imaging of ribosomes and RNA polymerase in live Escherichia coli cells. Mol. Microbiol. 85: 21–38 doi: 10.1111/j.1365-2958.2012.08081.x. [33] Fisher JK et al., 2013. Four-dimensional imaging of E. coli nucleoid organization and dynamics in living cells. Cell 153: 882–95 doi: 10.1016/j.cell.2013.04.006. [97] Sanamrad A et al., 2014. Single-particle tracking reveals that free ribosomal subunits are not excluded from the Escherichia coli nucleoid. PNAS 111: 11413–18 doi: 10.1073/pnas.1411558111.PubMed ID22624875, 23623305, 25056965
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| Comments |
p.364 bottom paragraph:"Antilandmarks also play roles in spatial patterning. For example, in E. coli, the nucleoid [the partly condensed form of the chromosome characteristic of many bacteria (ref 93)] occupies only ∼2–3% of the space in which it is present (primary source 33) yet, it spans ∼50–75% of the cytoplasmic volume (primary sources 6, 33, 97) and excludes polysomes (primary source 6). Since most ribosomes are part of polysomes, the nucleoid acts as an effective antilandmark for ribosomes. This exclusion is based on an entropic effect generally referred to as excluded volume. In this case, the presence of the nucleoid in part of the cell limits the local free volume available to polysomes." p.364 4th paragraph:"At the simplest level, a landmark is simply an object—such as a protein, lipid, or polymer—that already has a defined spatial distribution in the cell." |
| Entered by |
Uri M |
| ID |
112088 |