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p.5692 left column 3rd paragraph:"Irrespective of their size (100–1,500 cells), early wing imaginal discs show a constant proportion (average 1.4%) of TUNEL [terminal deoxynucleotidyltransferase-mediated UTP end labeling]-labeled cells (Fig. 1B) (except at the larval molts where there is a great increase in dying cells Fig. 1A and refs. 8 and 16)." P.5695 left column 2nd paragraph:"Apoptosis in the Wing Primordium: The pattern of appearance of apoptotic cells during normal development reveals certain regularities. Apoptotic cells occur as single cells or in clusters of neighboring cells with an average size of 3.4 cells, interestingly similar to that of clusters of cells synchronized in the same cell cycle stage (refs 16, 20). Clusters of apoptotic cells are also found in haltere and leg discs (unpublished results). Apoptotic cells remain in the epithelium for 2–4 h before being engulfed in place by hemolymph cells. Apoptosis in wild-type discs is a rather rare phenomenon during both larval and pupal development (1.4% of the anlage cells). At early larval stages apoptotic cells appear throughout the primordium but are topologically localized in characteristic regions at late stages (late larva and pupa). There is no cell death after 24 h APF [after puparium formation] at the end of the proliferative period of the wing primordium (ref 20)." |