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p.1273 left column bottom paragraph:"Cytonemes are dynamic structures: Next [investigators] analysed the behaviour of individual cytonemes. As in wing discs, Ihog–RFP/CFP expression stabilized cytonemes also in abdomen—[they] could observe individual protrusions for up to 9.5 h. To study cytoneme dynamics, [they] therefore used GMA [an actin-binding fragment of Moesin that labels actin filaments] instead of Ihog–RFP/CFP to visualize cytonemes. Without Ihog, histoblast cytonemes were thinner and shorter (up to 26 μm Fig. 5g,h and Supplementary Video S8) and had a lifetime of about 11±2.6 min (n = 6 Supplementary Video S9). With a growth rate of approximately 5 μm/min, they grew faster than filopodia observed in other in vivo systems (3.3 μm/min ref. 37) and in vitro assays (2.5 μm/min ref. 38). Cytonemes were very dynamic they grew to their final length and immediately shrank (Supplementary Fig. S3 and Video S10). [Investigators] could also image cytonemes using the membrane markers CD4–Tomato [ref 31] and Gap43–Venus [ref 39] (Supplementary Videos S11 and S12)." IHog=Hh co-receptor Interference hedgehog |