| Range |
a dense array of shorter cytonemes (~27µm long): individual longer cytonemes (≤70µm long) µm
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Bischoff M et al., Cytonemes are required for the establishment of a normal Hedgehog morphogen gradient in Drosophila epithelia. Nat Cell Biol. 2013 Nov15(11):1269-81. doi: 10.1038/ncb2856. p.1270 left column 4th paragraphPubMed ID24121526
|
| Method |
p.1270 left column 4th paragraph:"To analyse wing disc cytonemes [ref 28] in detail, [investigators] expressed fluorescently tagged Ihog in either A or P compartment cells." IHog=Hh co-receptor Interference hedgehog |
| Comments |
p.1270 left column 4th paragraph:"[Investigators] found that both cell types exhibited these processes basally (Fig. 1a–e). P compartment cells extended a dense array of shorter cytonemes (∼27 μm long) and individual longer cytonemes (up to 70 μm long) perpendicular to the A/P border into the neighbouring compartment. Cytonemes of A compartment cells were about 40–50% shorter than P compartment cytonemes. The processes were mostly linear (0.1–0.2 μm diameter), but some were branched, especially towards their distal end. The cytonemes covered most of the Hh gradient in the A compartment (7–12 cell diameters). [Investigators] could also detect lateral processes, but these were considerably shorter (1–2 cell diameters)." |
| Entered by |
Uri M |
| ID |
112075 |