| Range |
Table - link
|
| Organism |
Various |
| Reference |
Yamada H, Yamaguchi M, Chikamatsu K, Aono A, Mitarai S. Structome analysis of virulent Mycobacterium tuberculosis, which survives with only 700 ribosomes per 0.1 fl of cytoplasm. PLoS One. 2015 Jan 28 10(1):e0117109. doi: 10.1371/journal.pone.0117109. p.10/14 table 5PubMed ID25629354
|
| Primary Source |
[2] Biswas SK, Yamaguchi M, Naoe N, Takashima T, Takeo K (2003) Quantitative three-dimensional structural analysis of Exophiala dermatitidis yeast cells by freeze-substitution and serial ultrathin sectioning. J Electron Microsc 52: 133–143. [6] Yamaguchi M, Namiki Y, Okada H, Mori Y, Furukawa H (2011) Structome of Saccharomyces cerevisiae determined by freeze-substitution and serial ultrathin-sectioning electron microscopy. J Electron Microsc 60: 321–335. doi: 10.1093/jmicro/dfr052PubMed ID12868584, 21908548
|
| Method |
p.3 bottom paragraph:"Images obtained from negative scanning through Adobe Photoshop Elements (version 9) with CanoScan 8800F were saved as TIFF files and analyzed using ImageJ and Fiji software [refs 15, 16]. Briefly, cell length was calculated by multiplying the number by 55 nm (representing the thickness of each section). The diameter (minor and major axes), perimeter, and thickness of the plasma membrane (PM), outer membrane (OM), and cell envelope of each cell were measured as a pixel value using the line selection menu in the ImageJ/Fiji window as well as a scale bar recorded on the same negative. Measured pixel values were converted to μm or nm according to the measured pixel value of the scale bar on the corresponding negatives." |
| Comments |
p.9 bottom paragraph:"To date, there are only a few reports of quantitative structome analyses of microorganisms using TEM [transmission electron microscopy], including structome analyses of the yeast Exophiala dermatitidis and Saccharomyces cerevisiae [primary sources 2, 6]. These studies reported values for the average number of ribosomes per 0.1 fl of 1,100 (11,000/fl) and 1,950 (19,500/fl) for E. dermatitidis and S. cerevisiae, respectively. In the present study, [investigators] found an average density of ribosomes in M. tuberculosis cells of approximately 720/0.1 fl, a density that is about one-half and one-third that of E. dermatitidis and S. cerevisiae, respectively (Table 5). The reported ribosome densities for E. dermatitidis and S. cerevisiae [primary sources 2, 6] may be underestimates, as those studies enumerated ribosomes only in the cytoplasm and not in the nucleus, which comprises 7–10% of the cell volume." |
| Entered by |
Uri M |
| ID |
111990 |