Fraction of mRNA by mass

Value 4 %
Organism Bacteria Escherichia coli
Reference Moran MA et al., Sizing up metatranscriptomics. ISME J. 2013 Feb7(2):237-43. doi: 10.1038/ismej.2012.94. p.237 right column bottom paragraphPubMed ID22931831
Primary Source Neidhardt FC, Umbarger HE. (1996). Chemical composition of Escherichia coli. In: Neidhardt FC, Curtiss R III, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia Coli and Salmonella Typhimurium: Cellular and Molecular Biology, 2nd edn. ASM Press: Washington, DC, pp 13–16.
Comments p.237 right column bottom paragraph:"mRNA content of bacteria in natural environments can also be estimated based on the quantity of RNA recovered from a known number of cells. This calculation requires estimates of RNA extraction efficiency ([investigators] assumed 50%), the make-up of bacterial RNA ([they] assumed 4% mRNA by mass-primary source), and the average length of a bacterial mRNA ([they] assumed 924 nt BNID 111922)."
Entered by Uri M
ID 111921