| Range |
Table - link
|
| Organism |
BHK baby hamster kidney cells |
| Reference |
Quinn P, Griffiths G, Warren G. Density of newly synthesized plasma membrane proteins in intracellular membranes II. Biochemical studies. J Cell Biol. 1984 Jun98(6):2142-7. p.2144 table IPubMed ID6563038
|
| Method |
Abstract:"Using two independent methods, incorporation of radioactive amino-acid and quantitative immunoblotting, [investigators] have determined that the rate of synthesis of each of the Semliki Forest virus (SFV) proteins in infected baby hamster kidney (BHK) cells is 1.2 X 10^(5) copies/cell/min." |
| Comments |
p.2144 right column 2nd paragraph:”Subcellular fractions enriched in ER, Golgi, and plasma membranes were carbonate washed (ref 19) to remove luminal and peripheral proteins. The amounts of lipid and protein found are given in Table I. The mean molecular weight of the proteins in each fraction was calculated from scans of polyacrylamide gels. Each sample was run at several concentrations to allow estimation of each fraction at a loading that was within the range over which protein concentration and optical density on the film negative were linear. Representative scans are shown in Fig.5. The mean molecular weights and the consequent estimates of the density of membrane proteins are given in Table I.” See notes beneath table |
| Entered by |
Uri M |
| ID |
111709 |