| Range |
total 23 S type RNA ~2: total 16 S type RNA 3 - 5 %
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Lindahl, L. 1975. Intermediates and time kinetics of the in vitro assembly of Escherichia coli ribosomes. J. Mol. Biol. 92: 15–37. p.15 2nd paragraphPubMed ID1097701
|
| Method |
p.15 top paragraph:"The intermediates in the ribosome assembly in exponentially growing Escherichia coli have been identified by centrifuging a crude lysate, pulse-labeled with a radioactive RNA base, through a sucrose gradient and analyzing for precursor rRNA in the gradient fractions by gel electrophoresis." |
| Comments |
p.15 2nd paragraph:"The fraction of the rRNA contained in these precursor particles was determined by direct determination of the amount of rRNA in the precursor particles, and from the labeling kinetics of their rRNA. The direct estimation indicated that about 2% of the total 23 S type RNA, and 3 to 5% of the total 16 S type RNA is harboured in precursor particles." |
| Entered by |
Uri M |
| ID |
111680 |