Accumulation of RNA polymerase

Range Table - link
Organism Bacteria Escherichia coli
Reference Churchward G, Bremer H, Young R. Transcription in bacteria at different DNA concentrations. J Bacteriol. 1982 May150(2):572-81. p.575 table 2PubMed ID6175615
Method Abstract:"The effect of changing the DNA concentration on RNA synthesis, protein synthesis, and cell growth rate was studied in Escherichia coli B/r. The DNA concentration was varied by changing the replication velocity or by changing replication initiation in a thymine-requiring strain with a mutation in replication control."
Comments p.574 right column bottom paragraph:” RNA polymerase concentration and activity. The amount of RNA polymerase ß and ß' subunit protein was determined for E. coli B/r and TJK16 growing in glucose-amino acids medium at high and low thymine concentration (Table 2). Assuming that each subunit was part of a complete polymerase core enzyme, the fractional rate of accumulation of RNA polymerase, ap, was found to be about 1.33% (range, 1.29 to 1.35% [percentage of total protein that is RNA polymerase]) for both strains and independent of thymine concentration. Since mRNA and stable RNA synthesis per OD460 unit were the same for both strains (see below), it seems likely that also the concentrations of actively transcribing RNA polymerase molecules are the same.” See notes beneath table
Entered by Uri M
ID 111677