| Range |
height ~254 Å: width ~278 Å: thickness ~267 Å Å
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Verschoor A, Warner JR, Srivastava S, Grassucci RA, Frank J. Three-dimensional structure of the yeast ribosome. Nucleic Acids Res. 1998 Jan 15 26(2):655-61. p.656 left column paragraph above bottom paragraphPubMed ID9421530
|
| Method |
p.656 left column 4th paragraph:"The 80S ribosome from S.cerevisiae has been reconstructed from 7470 individual ribosome images to a resolution of 35 Å according to the Fourier Shell Criterion measure, using a critical value of 0.5 (ref 14), corresponding to a signal-to-noise ratio of ~2 (ref 15). This value closely agreed with the value obtained by the 45° phase residual criterion used in previous studies. Low pass filtration to 35 Å and, for correction of the transfer function, Wiener filtration were then applied." |
| Comments |
"The yeast ribosome is a bipartite structure that varies from roughly equidimensional to somewhat elongate depending on viewing angle (Fig. 1). In the orientation shown in Figure 1A the two subunits are seen side by side, 40S subunit on the left and 60S subunit on the right, with their ‘heads’ at the top. In this view the height of the ribosome is ~254 Å, the width ~278 Å and the thickness ~267 Å. These height and width dimensions are 11–14% smaller than those calculated for the ribosome from a higher eukaryote, wheatgerm (ref 3), but greater than those for the 70S E.coli ribosome (Fig. 1). That the thickness dimension is significantly greater for the yeast structure than for wheatgerm could be a result of experimental differences (see Discussion)." |
| Entered by |
Uri M |
| ID |
111542 |