H+/ATP ratio of mitochondrial & chloroplast F0F1 ATP Synthase

Range MF0F1 2.9±0.2: CF0F1 3.9±0.3 H+/ATP
Organism Various
Reference Petersen J, Förster K, Turina P, Gräber P. Comparison of the H+/ATP ratios of the H+-ATP synthases from yeast and from chloroplast. Proc Natl Acad Sci U S A. 2012 Jul 10 109(28):11150-5. doi: 10.1073/pnas.1202799109. p.11153 right column top paragraphPubMed ID22733773
Method p.11153 left column bottom paragraph:"In this work, the H+/ATP ratios of the yeast mitochondrial MF0F1 and of the chloroplast CF0F1 were measured in a simple chemiosmotic system, constituted by liposomes with the membrane-integrated enzymes."
Comments "The parallel measurement of H+/ATP for both MF0F1 and CF0F1 under identical experimental conditions in this high-precision system ensured that the n values obtained for both enzymes can be directly compared, in particular the imposed ?uH+ values, the stoichiometric product Q, and the ?G°'p were identical for both enzymes. [Investigators’] results were H+/ATP = 2.9 ± 0.2 for MF0F1 and H+/ATP = 3.9 ± 0.3 for CF0F1. The CF0F1 value is in agreement with the value 4.0 (± 0.2) reported earlier (refs 18, 19, 25, 26). As to MF0F1, different numbers, mainly 2 or 3, had been reported for the mammalian enzyme over the past decades (refs 27, 28 for review see ref. 29), but no data had been published for the yeast MF0F1." CF0F1 from chloroplasts: MF0F1 from yeast mitochondria
Entered by Uri M
ID 111521