Water activity, lag period, cell count, carbon dioxide production, oxygen absorption, maximal heat output (experimental and calculated)

Range Table - link
Organism Bacteria Escherichia coli
Reference Rothbaum HP, Stone HM. Heat output of Escherichia coli. J Bacteriol. 1961 Feb81: 172-7. p.175 table 3 (& p.173 table 1)PubMed ID13743863
Method p.172 left column 2nd paragraph:"For heat output measurements E. coli was grown in nutrient broth on the surface of asbestos fiber in order to obtain good aeration conditions." p.172 right column 2nd paragraph:"The temperature differential was continuously recorded on a 1 mv recorder and the outflowing air passed through a thermal conductivity type carbon dioxide analyzer, which recorded its concentration." p.172 right column 3rd paragraph:"Periodically, a small sample of asbestos was removed from the flask and the number of bacteria was estimated by vigorously shaking the sample with 100 ml of sterile Ringer's solution at 37°C for 2 min, and plating out at least three known dilutions on tryptone glucose extract agar medium (Difco)." p.173 left column top paragraph:"All heat output experiments were done in duplicate and never differed by more than 20 per cent." p.173 left column 2nd paragraph:"The water activity of broths was determined by a simple form of the isopiestic method of Robinson and Sinclair (1934)."
Comments "Table 3 tabulates all the results obtained. The lag period is taken to be the time before 1.0×10^-4 cal:sec:ml of heat is produced. All other results are given at the point of maximal heat output. These are cell counts, the percentage of coliform organisms on the plates, carbon dioxide production and oxygen consumption (both of which were always very close to a maximum at this point), measured heat output, calculated heat output (assuming that 1 g oxygen produces 3,600 cal), and heat output per cell. Experiments with broth containing 0.1 per cent mercuric chloride gave no heat output or carbon dioxide." For composition, density, and water activity of broths see table 1 beneath table 3.
Entered by Uri M
ID 111502