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Size of cell after recovery from hyperosmotic shock

Range	total length ~2.6: total radius ~0.97: cytoplasmic length ~2.2: cytoplasmic radius ~0.92 µm
Organism	Bacteria Escherichia coli
Reference	Pilizota T, Shaevitz JW. Fast, multiphase volume adaptation to hyperosmotic shock by Escherichia coli. PLoS One. 2012 7(4):e35205 doi: 10.1371/journal.pone.0035205. p.3 figure 1BPubMed ID22514721
Method	Values extracted visually from inset of figure 1B. "…[Investigators] sought to quantify cytoplasmic and periplasmic morphologies in living E. coli cells with high spatial and temporal resolution during osmotic shock and recovery (Figure 1). Briefly, [they] calculated the volume in these different cellular compartments by imaging a cytoplasmicly-expressed fluorescent protein [EGFP] and a fluorescent outer-membrane dye [FM4-64] either simultaneously or individually."
Comments	"In the first stage of shrinking, cell volume decreases in a few seconds and the cells adopt an hour-glass shape (Figure 1A, middle). During this phase, both the cell radius and length decrease (Figure 1B, insets). In the second stage, which lasts for several tens of seconds, the radius of the outer membrane increases with no significant length change (Figure 1B, inset). In contrast, while the cytoplasmic radius also increases, the cytoplasmic length decreases such that the cytoplasmic volume decreases only slightly, remaining roughly constant (Figure 1A, bottom right and Figure 1B, inset)."
Entered by	Uri M
ID	111480