Glucose consumption rate in proliferating fibroblasts

Range 0.5-5 nmol/µg protein/hour
Organism Human Homo sapiens
Reference Lemons JM et al., Quiescent fibroblasts exhibit high metabolic activity. PLoS Biol. 2010 Oct 19 8(10):e1000514. doi: 10.1371/journal.pbio.1000514. Supporting figure S1.APubMed ID21049082
Method Value was extracted visually from fig.S1A "[Investigators] developed a model system that allows [them] to monitor metabolic differences between proliferating and quiescent cells. Primary dermal fibroblasts were expanded and analyzed while actively proliferating, after 1 wk of growth to confluence (contact-inhibited for 7 d [CI7]), after 2 wk of confluence (contact-inhibited for 14 d [CI14]), or after 2 wk of confluence with serum concentrations decreased for the final week from 10% to 0.1% (contact-inhibited for 14 d and serum-starved for 7 d [CI14SS7]). Alternatively, fibroblasts were plated sparsely so that they did not touch each other and induced into quiescence by serum starvation and monitored after 4 d (serum-starved for 4 d [SS4]) or 7 d (serum-starved for 7 d [SS7])." "Rates of glucose consumption, lactate excretion, glutamine consumption, and glutamate excretion were monitored in proliferating, CI7, CI14, CI14SS7, SS4, SS7, proliferating low glucose/low glutamine, and CI14 low glucose/ low glutamine fibroblasts using the YSI 7100 bioanalyzer. Levels were normalized for the amount of cellular protein present during the conditioning time. Error bars indicate standard error."
Comments "Glucose consumption actually slightly increased in fibroblasts induced into quiescence by serum starvation (without contact inhibition) for 4 or 7 d (Figure S1)."
Entered by Uri M
ID 111474