| Range |
~205 µm/sec
|
| Organism |
Tetrahymena thermophila |
| Reference |
Wood CR, Hard R, Hennessey TM. Targeted gene disruption of dynein heavy chain 7 of Tetrahymena thermophila results in altered ciliary waveform and reduced swim speed. Wood CR, Hard R, Hennessey TM. J Cell Sci. 2007 Sep 1 120(Pt 17):3075-85. p.3079 fig.4APubMed ID17684060
|
| Method |
"The swim speed analysis was performed
in a total volume of 100 µl on a glass slide, with circular raised ring wells with an
inner diameter of 13 mm, outer diameter of 16 mm and depth of approximately 0.5
mm." Value extracted visually from fig.4A |
| Comments |
"To investigate whether the targeted gene disruption of DYH7
results in phenotypic defects in ciliary function, the swimming
speeds of mutant and wild-type cells were assayed and
compared. The mutant cell line, cultured in the presence of
selective agents, displayed a strong and consistent phenotype
in which swim speeds measured approximately half that of
wild type (Fig. 3 Fig. 4A, first two columns)." "Motility assays of both intact and reactivated models
of DYH7neo3 mutants revealed a stable, slow-swimming phenotype in which mutants displayed velocities of almost half that of wild-type cells (Figs 4 and 7)." |
| Entered by |
Uri M |
| ID |
111436 |