| Range |
56 - 84 %
|
| Organism |
Mouse Mus musculus |
| Reference |
Li et al. (2014), System wide analyses have underestimated protein abundances and the importance of
transcription in mammals. PeerJ 2:e270 DOI 10.7717/peerj.270 abstract & p.16 2nd paragraphPubMed ID24688849
|
| Primary Source |
Schwanhausser et al., 2011. Global quantification of mammalian gene expression control. Nature 473: 337–342 DOI 10.1038/nature10098.PubMed ID21593866
|
| Method |
"Using individual measurements for 61 housekeeping proteins to rescale whole proteome data from Schwanhausser et al. (2011 primary source), [researchers] find that the median protein detected is expressed at 170,000 molecules per cell and that [their] corrected protein abundance estimates show a higher correlation with mRNA abundances than do the uncorrected protein data. In addition, [researchers] estimated the impact of further errors in
mRNA and protein abundances using direct experimental measurements of these
errors. The resulting analysis suggests that mRNA levels explain at least 56% of
the differences in protein abundance for the 4,212 genes detected by Schwanhausser
et al. (2011 primary source), though because one major source of error could not be estimated the
true percent contribution should be higher. [Researchers] also employed a second, independent
strategy to determine the contribution of mRNA levels to protein expression. [Researchers]
show that the variance in translation rates directly measured by ribosome profiling is
only 9% of that inferred by Schwanhausser et al. (2011 primary source), and that the measured and
inferred translation rates correlate poorly (R^2 = 0.14). Based on this, [researchers'] second
strategy suggests that mRNA levels explain ~84% of the variance in protein levels." |
| Comments |
Please note-"By measuring
mRNA degradation and protein degradation rates as well, the rates of transcription and
translation can be additionally inferred indirectly. Using this approach to study mouse
NIH3T3 fibroblasts, Schwanhausser et al. (2011 primary source) concluded that mRNA levels explain
~40% of the variability in protein levels. That the cellular abundance of proteins is
predominantly controlled at the level of translation. That transcription is the second largest
determinant. And that the degradation of mRNAs and proteins play a significant but lesser
role." "...[researchers] estimate that
mRNA abundance explains 56%–84% for a set of 4,212 detected proteins." Primary source: "[Researchers] used parallel metabolic pulse labelling with amino acids and 4sU
to measure simultaneously protein and mRNA turnover in a population of exponentially growing non-synchronized NIH3T3 mouse
fibroblasts (Fig. 1a)" |
| Entered by |
Uri M |
| ID |
111364 |