| Value |
64
nm
Range: ±15 nm
|
| Organism |
Chlamydomonas reinhardtii |
| Reference |
Engel, B. D., Schaffer, M., Kuhn Cuellar, L., Villa, E., Plitzko, J. M., and Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. Elife 4, 1–29. p.8 bottom paragraphPubMed ID25584625
|
| Primary Source |
Greenwood AD, Leech RM, Williams JP. 1963. The osmiophilic globules of chloroplasts: I. Osmiophilic globules as a normal component of chloroplasts and their isolation and composition in Vicia faba L. Biochimica Et Biophysica Acta 78: 148–162. doi: 10.1016/0006-3002(63)91620-2. & Ohad I, Siekevitz P, Palade GE. 1967. Biogenesis of chloroplast membranes. II. Plastid differentiation during greening of a dark-grown algal mutant (Chlamydomonas reinhardii). The Journal of Cell Biology 35: 553–584.doi: 10.1083/jcb.35.3.553.PubMed ID6064365
|
| Method |
in situ cryo-electron tomography |
| Comments |
"The cluster of RuBisCO complexes in the pyrenoid was encased in a starch sheath (Figures 1G, 3A–B
and 9A–B), and non-spherical starch granules were found throughout the chloroplast (Figures 1G,
3A–B, 4A–B and 7A–B). In addition to starch, [researchers] observed smaller 64 ± 15 nm spherical plastoglobules
(n = 20 globules from 9 tomograms) (primary sources) that were
always closely associated with thylakoid membranes (Figure 4)." |
| Entered by |
Uri M |
| ID |
111330 |