| Range |
Table - link
|
| Organism |
Bacteria Geobacter metallireducens |
| Reference |
Feist AM. et al., Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. PLoS Comput Biol. 2014 Apr 24 10(4):e1003575. doi: 10.1371/journal.pcbi.1003575. p.7 table 3PubMed ID24762737
|
| Method |
"An updated reconstruction of G. metallireducens GS-15, iAF987,
was generated by reconciling an existing genome-scale reconstruction
[13] and an updated genome annotation, performing a bottomup
reconstruction of additional metabolic pathways." |
| Comments |
"The first step in the modeling process was to examine the
fumarate reductase reaction and maintenance energies necessary
for predicting phenotypes using constraint-based analysis (Step 1,
Figure 3C). To examine this content, data was generated using the
G. metallireducens dcuB strain and utilized in simulations. An
electrogenic bifunctional fumarate reductase/succinate dehydrogenase
(FRD2rpp) was included based on recent biochemical
evidence in a similar species [15]. This inclusion was validated
with the growth data of G. metallireducens on acetate and fumarate
using the dcuB strain. The earlier version of the G. metallireducens
model [13] and the current model (iAF987) without an electrogenic
fumarate reductase were unable to produce a feasible solution when constrained with experimentally measured acetate
and fumarate uptake rates (see Table 3)." |
| Entered by |
Uri M |
| ID |
111307 |