| Value |
1.14
hour^-1
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Selvarasu S. et al., Characterizing Escherichia coli DH5alpha growth and metabolism in a complex medium using genome-scale flux analysis. Biotechnol Bioeng. 2009 Feb 15 102(3):923-34. doi: 10.1002/bit.22119. p.926 right column top paragraphPubMed ID18853410
|
| Method |
"Finally, the cellular objective of the cell growth rate during
the growing phase was maximized using linear programming
(LP), thereby resulting in a set of metabolic flux
distribution corresponding to the optimal phenotype
(Edwards et al., 2002). In the current work, the LP problem
was solved using a stand-alone flux analysis program,
MetaFluxNet (Lee et al., 2003). The specific growth rate
obtained from the optical density values (OD600) measurements
during the exponential growth phase was compared
with the cell growth predicted by the in silico model to
validate results...Batch cultures of E. coli DH5a cells were conducted in 5-L
vessels provided with a complex nutrient medium." |
| Comments |
"The in silico prediction of a cell growth rate of 1.07 h^-1
during the exponential growth phase (phase 1) was close
(within the acceptable error range of 10%) to the
experimentally observed specific growth rate of 1.14 h^-1." |
| Entered by |
Uri M |
| ID |
111284 |