Transcription error rate throughout ~450-bp cDNA of CAN1 transcript

Value 1.3e-3 substitution/bp
Organism Yeast
Reference Imashimizu M, Oshima T, Lubkowska L, Kashlev M. Direct assessment of transcription fidelity by high-resolution RNA sequencing. Nucleic Acids Res. 2013 Oct41(19):9090-104. doi: 10.1093/nar/gkt698.PubMed ID23925128
Primary Source Nesser,N.K., Peterson,D.O. and Hawley,D.K. (2006) RNA polymerase II subunit Rpb9 is important for transcriptional fidelity in vivo. Proc. Natl Acad. Sci. USA, 103, 3268–3273.PubMed ID16492753
Method Primary source abstract:"The fidelity of yeast RNA polymerase II (Pol II) was assessed in vivo with an assay in which errors in transcription of can1–100, a nonsense allele of CAN1, result in enhanced sensitivity to the toxic arginine analog canavanine."
Comments "In eukaryotic transcription, Nesser et al. previously analyzed transcription errors throughout ~450-bp cDNA of CAN1 transcript in yeast by Sanger sequencing (primary source). Their work reported a much higher 1.3×10^-3/bp rate of substitutions compared to the rate observed for E. coli RNAP in [researchers'] study and the rates determined for yeast RNAP II in vitro (Kireeva et al. 2008 PMID 18538654)."
Entered by Uri M
ID 111145